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European Archives of Paediatric Dentistry

09.05.2024 | Original Scientific Article

Evaluating antimicrobial activity and cytotoxicity of silver nanoparticles incorporated into reinforced zinc oxide eugenol: an in vitro study

verfasst von: T. S. Shid-Moosavi, N. Mohammadi, Y. Gharamani, M. Motamedifar, A. A. Alizadeh

Erschienen in: European Archives of Paediatric Dentistry

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Abstract

Purpose

This study aimed to evaluate the antibacterial and cytotoxic effects of reinforced zinc oxide–eugenol (rZOE) incorporated with different concentrations of silver nanoparticles (AgNPs).

Methods

The pastes of rZOE alone or mixed with AgNPs at concentrations of 1%, 2%, and 5% of weight were prepared. In vitro antimicrobial activity of prepared materials against Streptococcus (S.) mutans and Lactobacillus (L.) acidophilus were evaluated after 2, 4, and 6 h of contact times using direct contact test (DCT) and also following 24 h incubation by well-diffusion test (WDT). The cytotoxicity of the tested materials on human dental pulp stem cells was also determined by MTT assay.

Results

The DCT demonstrated that the time-dependent reductions of the colony numbers of both bacteria by three different concentrations of AgNPs incorporated into rZOE were equal but steeper than the rZOE alone (P < 0.05). The increases in growth inhibition zones of S. mutans and L. acidophilus were associated with the increasing concentration of AgNPs mixed with rZOE in the WDT; however, statistical analysis did not show any significant differences (P = 0.092). The MTT assay revealed a significantly lower percentage of cell viability after 1 day of culture only with the rZOE + AgNP5% in comparison to the rZOE alone (P = 0.011) and the control medium (P = 0.001).

Conclusion

Since the antimicrobial activities of three different concentrations of AgNPs incorporated into rZOE were equal and AgNPs had lower toxicity at lower concentrations, using AgNPs at 1% concentration is suggested to be mixed with rZOE.

Alzaidy FA, Khalifa AK, Emera RM. The antimicrobial efficacy of nanosilver modified root canal sealer. Eur J Res Med Sci. 2018;6(1):1–6.

AshaRani P, Low Kah Mun G, Hande MP, Valiyaveettil S. Cytotoxicity and genotoxicity of silver nanoparticles in human cells. ACS Nano. 2009;3(2):279–90. https://doi.org/10.1021/nn800596w.CrossRefPubMed

Bahador A, Pourakbari B, Bolhari B, Hashemi FB. In vitro evaluation of the antimicrobial activity of nanosilver-mineral trioxide aggregate against frequent anaerobic oral pathogens by a membrane-enclosed immersion test. Biomed J. 2015;38(1):77–83. https://doi.org/10.4103/2319-4170.132901.CrossRefPubMed

Bedier MMA. Antibacterial efficacy of mineral trioxide aggregate combined with nano-silver additives. Egypt Dental J. 2017;63(2):1833–41. https://doi.org/10.21608/EDJ.2017.75138.CrossRef

Bossù M, Iaculli F, Di Giorgio G, Salucci A, Polimeni A, Di Carlo S. Different pulp dressing materials for the pulpotomy of primary teeth: a systematic review of the literature. J Clin Med. 2020;9(3):838. https://doi.org/10.3390/jcm9030838.CrossRefPubMedPubMedCentral

Cox ST Jr, Hembree JH Jr, McKnight JP. The bactericidal potential of various endodontic materials for primary teeth. Oral Surg Oral Med Oral Pathol. 1978;45(6):947–54. https://doi.org/10.1016/s0030-4220(78)80017-6.CrossRefPubMed

Duque C, de Cassia Negrini T, Sacono NT, Spolidorio DMP, de Souza Costa CA, Hebling J. Clinical and microbiological performance of resin-modified glass-ionomer liners after incomplete dentine caries removal. Clin Oral Investig. 2009;13(4):465–71. https://doi.org/10.1007/s00784-009-0304-2.CrossRefPubMed

Eskandarian T, Motamedifar M, Arasteh P, Eghbali SS, Adib A, Abdoli Z. Comparison of antimicrobial effects of titanium tetrafluoride, chlorhexidine, xylitol and sodium fluoride on streptococcus mutans: an in-vitro study. Electr Phys. 2017;9(3):4042–7. https://doi.org/10.19082/4042.CrossRef

Foldbjerg R, Olesen P, Hougaard M, Dang DA, Hoffmann HJ, Autrup H. PVP-coated silver nanoparticles and silver ions induce reactive oxygen species, apoptosis and necrosis in THP-1 monocytes. Toxicol Lett. 2009;190(2):156–62. https://doi.org/10.1016/j.toxlet.2009.07.009.CrossRefPubMed

Fuks A, Papagiannoulis L, Duggal M. Pulpotomy in primary teeth: review of the literature according to standardized assessment criteria. Eur Arch Paediatr Dent. 2006;7(2):64–71. https://doi.org/10.1007/BF03320817.CrossRefPubMed

Gomes-Filho JE, Silva FO, Watanabe S, Cintra LT, Tendoro KV, Dalto LG, Pacanaro SV, Lodi CS, de Melo FF. Tissue reaction to silver nanoparticles dispersion as an alternative irrigating solution. J Endod. 2010;36(10):1698–702. https://doi.org/10.1016/j.joen.2010.07.007.CrossRefPubMed

Javidi M, Zarei M, Omidi S, Ghorbani A, Gharechahi M, Rad MS. Cytotoxicity of a new nano zinc-oxide eugenol sealer on murine fibroblasts. Iran Endod J. 2015;10(4):231–5. https://doi.org/10.7508/iej.2015.04.004.CrossRefPubMedPubMedCentral

Jha S, Goel N, Dash BP, Sarangal H, Garg I, Namdev R. An Update on newer pulpotomy agents in primary teeth: A literature review. J Pharm Bioallied Sci. 2021;13(Suppl 1):S57–61. https://doi.org/10.4103/jpbs.JPBS_799_20.CrossRefPubMedPubMedCentral

Jun SK, Kim HW, Lee HH, Lee JH. Zirconia-incorporated zinc oxide eugenol has improved mechanical properties and cytocompatibility with human dental pulp stem cells. Dent Mater. 2018;34(1):132–42. https://doi.org/10.1016/j.dental.2017.09.021.CrossRefPubMed

Lebda MA, Sadek KM, Tohamy HG, Abouzed TK, Shukry M, Umezawa M, El-Sayed YS. Potential role of α-lipoic acid and Ginkgo biloba against silver nanoparticles-induced neuronal apoptosis and blood-brain barrier impairments in rats. Life Sci. 2018;212:251–60. https://doi.org/10.1016/j.lfs.2018.10.011.CrossRefPubMed

Li L, Li L, Zhou X, Yu Y, Li Z, Zuo D, Wu Y. Silver nanoparticles induce protective autophagy via Ca2+/CaMKKβ/AMPK/mTOR pathway in SH-SY5Y cells and rat brains. Nanotoxicology. 2019;13(3):369–91. https://doi.org/10.1080/17435390.2018.1550226.CrossRefPubMed

Liao C, Li Y, Tjong SC. Bactericidal and cytotoxic properties of silver nanoparticles. Int J Mol Sci. 2019;20(2):449. https://doi.org/10.3390/ijms20020449.CrossRefPubMedPubMedCentral

Maltz M, Oliveira E, Fontanella V, Carminatti G. Deep caries lesions after incomplete dentine caries removal: 40-month follow-up study. Caries Res. 2007;41(6):493–6. https://doi.org/10.1159/000109349.CrossRefPubMed

Moskovitz M, Tickotsky N, Dassa M, Fux-Noy A, Shmueli A, Halperson E, Ram D. Zinc oxide zinc sulfate versus zinc oxide eugenol as pulp chamber filling materials in primary molar pulpotomies. Children. 2021;8(9):776. https://doi.org/10.3390/children8090776.CrossRefPubMedPubMedCentral

Navit S, Jaiswal N, Khan SA, Malhotra S, Sharma A, Mukesh JS, Agarwal G. Antimicrobial efficacy of contemporary obturating materials used in primary teeth-an in-vitro study. J Clin Diagn Res. 2016. https://doi.org/10.7860/JCDR/2016/21883.8426.CrossRefPubMedPubMedCentral

Parisay I, Ghoddusi J, Forghani M. A review on vital pulp therapy in primary teeth. Iran Endod J. 2015;10(1):6–15.PubMed

Park MV, Neigh AM, Vermeulen JP, de la Fonteyne LJ, Verharen HW, Briedé JJ, van Loveren H, de Jong WH. The effect of particle size on the cytotoxicity, inflammation, developmental toxicity and genotoxicity of silver nanoparticles. Biomaterials. 2011;32(36):9810–7. https://doi.org/10.1016/j.biomaterials.2011.08.085.CrossRefPubMed

Parsaee F, Alizadeh A, Rezaee M, Alavi O, Alipour H. Evaluation of the osteoconductive properties of scaffold containing platete-enriched-fibrin (PRF) with three calcium phosphate (TCP) in the alveolar socket repair after tooth extraction: An animal study. J Biomater Appl. 2023;37(10):1789–800. https://doi.org/10.1177/08853282231170346.CrossRefPubMed

Pimenta HC, Borges ÁH, Bandeca MC, Neves ATS, Fontes RG, da Silva PV, Aranha AM. Antimicrobial activity of filling materials used in primary teeth pulpotomy. J Int Oral Health. 2015;7(4):54–7.PubMedPubMedCentral

Poggio C, Arciola CR, Beltrami R, Monaco A, Dagna A, Lombardini M, Visai L. Cytocompatibility and antibacterial properties of capping materials. Sci World J. 2014;2014: 181945. https://doi.org/10.1155/2014/181945.CrossRef

Samiei M, Aghazadeh M, Lotfi M, Shakoei S, Aghazadeh Z, Pakdel SMV. Antimicrobial efficacy of mineral trioxide aggregate with and without silver nanoparticles. Iran Endod J. 2013;8(4):166–70.PubMedPubMedCentral

Seung J, Weir MD, Melo MAS, Romberg E, Nosrat A, Xu HHK, Tordik PA. A modified resin sealer: physical and antibacterial properties. J Endod. 2018;44(10):1553–7. https://doi.org/10.1016/j.joen.2018.06.016.CrossRefPubMed

Shalhav M, Fuss Z, Weiss EI. In vitro antibacterial activity of a glass ionomer endodontic sealer. J Endod. 1997;23(10):616–9. https://doi.org/10.1016/S0099-2399(97)80172-0.CrossRefPubMed

Sherief DI, Fathi MS, Abou El Fadl RK. Antimicrobial properties, compressive strength and fluoride release capacity of essential oil-modified glass ionomer cements —an in vitro study. Clin Oral Investig. 2021;25(4):1879–88. https://doi.org/10.1007/s00784-020-03493-0.CrossRefPubMed

Shirali S, Javadinejad S, Tahmoraspour A. Antimicrobial effect of zinc oxide eugenol mixed with nanosilver on commonly isolated bacteria from the primary root canals with necrotic pulp. J Isfahan Dent Sch. 2020;16(3):254–63.

Siqueira JF Jr, Favieri A, Gahyva SM, Moraes SR, Lima KC, Lopes HP. Antimicrobial activity and flow rate of newer and established root canal sealers. J Endod. 2000;26(5):274–7. https://doi.org/10.1097/00004770-200005000-00005.CrossRefPubMed

Siqueira PC, Magalhães AP, Pires WC, Pereira FC, Silveira-Lacerda EP, Carrião MS, Bakuzis AF, Souza-Costa CA, Lopes LG, Estrela C. Cytotoxicity of glass ionomer cements containing silver nanoparticles. J Clin Exp Dent. 2015;7(5):e622–7. https://doi.org/10.4317/jced.52566.CrossRefPubMedPubMedCentral

Tobias R. Antibacterial properties of dental restorative materials: a review. Int Endod J. 1988;21(2):155–60. https://doi.org/10.1111/j.1365-2591.1988.tb00969.x.CrossRefPubMed

Yalcin M, Arslan U, Dundar A. Evaluation of antibacterial effects of pulp capping agents with direct contact test method. Eur J Dent. 2014;8(1):95–9. https://doi.org/10.4103/1305-7456.126256.CrossRefPubMedPubMedCentral

Yin IX, Yu OY, Zhao IS, Mei ML, Li Q-L, Tang J, Chu C-H. Developing biocompatible silver nanoparticles using epigallocatechin gallate for dental use. Arch Oral Biol. 2019;102:106–12. https://doi.org/10.1016/j.archoralbio.2019.03.022.CrossRefPubMed

Yin IX, Zhang J, Zhao IS, Mei ML, Li Q, Chu CH. The antibacterial mechanism of silver nanoparticles and its application in dentistry. Int J Nanomed. 2020;15:2555–62. https://doi.org/10.2147/IJN.S246764.CrossRef

Zand V, Lotfi M, Aghbali A, Mesgariabbasi M, Janani M, Mokhtari H, Tehranchi P, Pakdel SM. Tissue reaction and biocompatibility of implanted mineral trioxide aggregate with silver nanoparticles in a rat model. Iran Endod J. 2016;11(1):13–6. https://doi.org/10.7508/iej.2016.01.003.CrossRefPubMed

Titel: Evaluating antimicrobial activity and cytotoxicity of silver nanoparticles incorporated into reinforced zinc oxide eugenol: an in vitro study
verfasst von: T. S. Shid-Moosavi
N. Mohammadi
Y. Gharamani
M. Motamedifar
A. A. Alizadeh
Publikationsdatum: 09.05.2024
Verlag: Springer Berlin Heidelberg
Erschienen in: European Archives of Paediatric Dentistry
Print ISSN: 1818-6300
Elektronische ISSN: 1996-9805
DOI: https://doi.org/10.1007/s40368-024-00905-7

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Evaluating antimicrobial activity and cytotoxicity of silver nanoparticles incorporated into reinforced zinc oxide eugenol: an in vitro study

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